You accidentally added the poe8 forward and reverse primers instead of the 165 rRNA primers Your instructor didn't add Taq polymerase to your PCR tube Question 5 1 pts Q5. Oniy one of a given peimer pair was added to the reaction D R Your instructor didn't add Taq polymerase to your PCR tube The colony you piched fram actually contained a number of different Aliivibrio species 5YBR Sate dye was not added to the gel Only one of a given primer pair was added to the reaction. (Two sclective canditions - SWC growth and bioluminescenti. Note the primers chosen (both the rpoB and the 165 rRNA primers) are fairly universal which means they should anncal to marine bacterial species that are biolumincscent. Select the best choice that includes the most possible reasonable explanations. Some list more than one possible explanation. A list of possible reasons are provided as possible answers. There were other bands in the sarne gel for other students' PCRs (165 and rpoB), including the positive controls. You do agarose gel electrophoresis for your PCR reaction and see no bands for your 165 rRNA.

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